Novel genetic tools for Hansenula polymorpha
نویسندگان
چکیده
منابع مشابه
Pexophagy in Hansenula polymorpha.
In the yeast Hansenula polymorpha the development and turnover of peroxisomes is readily achieved by manipulation of the cultivation conditions. The organelles massively develop when the cells are incubated in the presence of methanol as the sole source of carbon and energy. However, they are rapidly and selectively degraded when methanol-grown cells are placed at conditions of repression of me...
متن کاملHansenula polymorpha Vam7p is required for macropexophagy.
We have analyzed the functions of two vacuolar t-SNAREs, Vam3p and Vam7p, in peroxisome degradation in the methylotrophic yeast Hansenula polymorpha. A Hp-vam7 mutant was strongly affected in peroxisome degradation by selective macropexophagy as well as non-selective microautophagy. Deletion of Hp-Vam3p function had only a minor effect on peroxisome degradation processes. Both proteins were loc...
متن کاملMethanol oxidation and assimilation in Hansenula polymorpha
The metabolism of methanol was monitored in whole cells of the methylotrophic yeast Hansenula polymorpha by using [13C]methanol and n.m.r. in vivo. The main products observed under normal conditions were trehalose and glycerol, whereas cells that were starved before exposure to [13C]methanol also accumulated glutamate, glutamine and alanine; formate was also more prominent in spectra from starv...
متن کاملCreation of Hansenula polymorpha fatty acid auxotrophic mutants for genetic study of fatty acid biosynthesis
Three groups of Hansenula polymorpha fatty acid auxotrophic mutants were screened by their fatty acid requirement for growth. The mutants are saturated fatty acid auxotrophic mutants (Sfa), monounsaturated fatty acid auxotrophic mutants (Mfa) and polyunsaturated fatty acid auxotrophic mutants (Pfa) which required saturated fatty acids, monounsaturated fatty acids and polyunsaturated fatty acids...
متن کاملThe Hansenula polymorpha PEX14 gene encodes a novel peroxisomal membrane protein essential for peroxisome biogenesis.
We have cloned the Hansenula polymorpha PEX14 gene by functional complementation of the chemically induced pex14-1 mutant, which lacked normal peroxisomes. The sequence of the PEX14 gene predicts a novel protein product (Pex14p) of 39 kDa which showed no similarity to any known protein and lacked either of the two known peroxisomal targeting signals. Biochemical and electron microscopical analy...
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ژورنال
عنوان ژورنال: FEMS Yeast Research
سال: 2011
ISSN: 1567-1356
DOI: 10.1111/j.1567-1364.2011.00772.x